Abstract: Diabetes mellitus is affecting human health all over the world. Both environmental and genetic factors are implicated in its pathogenesis. However, the primary of the factors are still debated. Biochemical mechanism(s) in pancreatic b-cell destructions rendering reduced insulin secretion is postulated to be associated with genetic variation and hence the present proposal was aimed to explore insulin secretory capacity (HOMA-%B), insulin sensitivity (HOMA-%S) and total antioxidant status (TAS) in subjects having parental history of type 2 diabetes mellitus (T2DM) and compared with their negative counterpart.
In this cross-sectional study, 96 normoglycemic subjects were enrolled, among them 48 subjects (case) had parental history of T2DM, rest 48 without parental history of T2DM (controls). Blood glucose was measured by glucose oxidase method. Serum total cholesterol, triglycerides and high density lipoprotein (HDL) cholesterol were measured using Dimension®RxL max clinical chemistry system, and serum low density lipoprotein cholesterol (LDL) were calculated by Friedewald formula. Serum insulin was measured by enzyme linked immunosorbent assay. HOMA-%B, HOMA-%S and insulin resistance (HOMA IR) were assessed from fasting glucose and insulin using HOMA2 calculator. Total antioxidant status (TAS) was determined by spectrophotometric technique. Data were expressed as median (range) and Mann Whitney U test was performed to calculate statistical difference between groups using GraphPad Prism (6.02). P value < 0.05 was taken as level of significance.
Median (range) of age (years) was 35.0 (30–50) and 36.5 (30–49) in case and controls respectively. Median (range) of fasting and 2hrs plasma glucose (mmol/L) was 4.9 (3.4–7.7) and 5.9 (4.4–10.6) in case and 4.9 (3.5–5.9) and 5.8 (3.8–7.8) in control respectively. Median (range) of fasting serum insulin (μIU/mL) was 16.1 (4.8–53.2) and 13.3 (2.5–46.3) in case and controls which showed statistical difference (p = 0.0134). HOMA-%B was significantly higher in case compared to control [179 (55–381) vs 98 (56–335), p = 0.001]. HOMA %S was significantly lower [49.3 (17.2–95.8) vs 60.2 (15.5–152.9), p = 0.0365] and HOMA IR was significantly higher [2.0 (1.0–5.8) vs 1.7 (0.7– 6.5), p = 0.0365] in case compared to control subjects with BMI ≤ 25 Kg/m². No significance difference was observed in TAS between case and control group [1296 (579 – 2164) vs 1549 (737 – 2690) μmol/L, p = 0.1330].
Data concluded that normoglycemic offspring of T2DM subjects had higher insulin secretory capacity as evidenced by absolute circulating insulin level and HOMA-%B as a measure of pancreatic b-cell secretion suggesting possible influence on heightened expression of related genes. The plausible mechanism(s) are yet to be identified. Similar levels of total antioxidant status (TAS) in the two groups preclude the oxidative stress involved in the process.
Keywords: Antioxidant status, diabetes, insulinaemic, normoglycemic offspring.
Title: Insulinemic and antioxidant status in normoglycemic subjects with parental history of type 2 diabetes mellitus
Author: Md. Ehsanul Islam khan, Biplob Chandra Shil
International Journal of Healthcare Sciences
ISSN 2348-5728 (Online)
Vol. 10, Issue 1, April 2022 - September 2022
Page No: 15-20
Research Publish Journals
Website: www.researchpublish.com
Published date: 21-April-2022
