Abstract: Objective is to investigate the total phenols, flavonoids, carotenoids, antioxidant, antimicrobial and cytotoxic activites of Samadera indica flower and flower stalk extracts and also to investigate components of essential oils from flower and flower stalk of Samadera indica.
Method: Total phenols, flavonoids, carotenoids content, DPPH scavenging activity, reducing power activity, phosphomolybdenum activity, metal chelating activity, hydrogen peroxide and hydroxyl radical scavenging activity of crude extracts, cytotoxic activity, antibacterial screening were evaluated. The extraction of essential oils by steam distillation and GCMS data were analyzed.
Results: The total phenols, flavonoids and carotenoids of Samadera indica methanol extract of flower and flower stalk were determined and were found to be 96.64 ± 0.136 mg catechol equ per gram, 119.81 ± 0.236 mg quercetin equ per gram and 80.46 ± 0.135 mg β-carotene equ per gram for flower and 87.69151 ± 0.15 mg catechol equ per gram, 104.48 ± 0.136 mg quercetin equ per gram and 65.71 ± 0.581 mg β-carotene equ per gram for flower stalk. The antioxidant activity of the petroleum ether, ethyl acetate, chloroform, methanol and aqueous extracts of Samadera indica flower and flower stalk were investigated using reducing power assay, metal chelating assay, DPPH radical scavenging assay, Hydrogen peroxide radical scavenging assay, Hydroxyl radical scavenging assay and phosphomolybdenum assay. Methanol extracts of flower and flower stalk showed more activity on DPPH assay, reducing power assay and metal chelating activity assay than other extracts and their activity increased with increasing concentration of extracts. The antioxidant activity of the flower and flower stalk methanol extracts were found to be 96.314 and 104.18 μg/ml for hydrogen peroxide scavenging assay, 115.28 and 135.96 mg equivalent of ascorbic acid/100 g of plant extract by phosphomolybdenum method. The hydroxyl radical scavenging activity of methanol extracts are quite comparable to the standard Mannitol of the assay. The antibacterial studies on various extracts of flower and flower stalk of plant showed that methanol extracts showed good inhibitory activity against all the test pathogens compared with standard antibiotics streptomycin and penicillin.The remarkable cytotoxic activity showed by flower and flower stalk methanol extracts could be attributed to the synergic effect of the active compounds present in them.
Conclusions: The presence of major bioactive compound, phytol in flower essential oil and 1,2-Benzenedicarboxylic acid, butyl methyl ester in flower stalk essential oil revealed to use flower and flower stalk (pedicel and peduncle) of the plant in drug delivery, nutritional or pharmaceutical fields.
Keywords: Flower and flower stalk of Samadera indica, GC-MS analysis, bioactive compounds.
Title: INVESTIGATION ON ANTIOXIDANT, ANTIBACTERIAL, CYTOTOXIC POTENTIAL AND ESSENTIAL OILS OF FLOWER AND FLOWER STALK OF SAMADERA INDICA FROM SOUTH INDIA
Author: Sindhu C, Beena Jose
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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