Isolation and Characterization of Actinomycetes from Soil and Screening Their Antibacterial Activities against Different Microbial Isolates

Jyoti Tyagi, Tripti Bhatnagar, Dr. Fanish Kumar Pandey

Abstract: A total 11 Actinomycetes strains were isolated from the Noida.  Soil sample had been screened for their antibacterial activity. They were evaluated for their inhibitory activities on 4 tests Organism. The culture characteristics of isolates were also studies in different culture media. The results indicated that 11 Actinomycetes isolates were highly active against Staphylococcusaureus and Pseudomonasaeruginosa, Enterobacter, Streptococcus strains. 11 Actinomycetes isolates were highly active with an inhibition zone more than 20 mm in diameter. Most of the isolates inhibited growth of the Gram negative bacteria tested. All the antibiotic producing actinomycetes were isolated at 28°C from soil. 11 Actinomycetes isolates showed activity against bacteria in which most of them from alkaline soil. Where the less interference by human for agriculture or other purpose. These micro-organisms may have capability to produce some of the most important medicines ever developed.

Keywords: Antibacterial activity, Antibiotics drugs resistance, Soil sample.

Title: Isolation and Characterization of Actinomycetes from Soil and Screening Their Antibacterial Activities against Different Microbial Isolates

Author:  Jyoti Tyagi, Tripti Bhatnagar, Dr. Fanish Kumar Pandey

International Journal of Life Sciences Research

ISSN 2348-3148 (online), ISSN2348-313X (Print)

Research Publish Journals

Vol. 2, Issue 4, October 2014 - December 2014

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Isolation and Characterization of Actinomycetes from Soil and Screening Their Antibacterial Activities against Different Microbial Isolates by Jyoti Tyagi, Tripti Bhatnagar, Dr. Fanish Kumar Pandey