Abstract: The aim of the study was to isolate a potent microbial strain as an enzymatic source for textile biobleaching. The experiment isolated a fungal strain resembling Aspergillus niger from soil contaminated with textile effluents. The fungal strain exhibited decolouration of reactive dyes on solid culture media and was further analyzed in liquid state fermentation to study various production parameters. The enzyme of interest is an extracellular enzyme showing maximum decolourization at 96 hrs of fermentation cycle. The active enzyme has optimum substrate concentration of glucose at 20gm/lt and optimum pH: 5.0. The active enzyme has been identified to be an inducible Glucose Oxidase enzyme exhibiting GOX activity 3.1u/ml in the crude broth and has been partially purified using ammonium sulfate precipitation. The crude enzyme has been applied to study the biobleaching of cotton grey fabric and has shown visible bleaching effect in the experiments. The biobleaching has been validated using authentic methods at The Bombay Textile Research Association Laboratories, Bombay. The research showed that the bio-bleaching system has a powerful bleaching ability under mild process conditions and that it has the future prospect of industrial application in decreasing the environment effluent load and in water preservation.
Keywords: microbial enzyme, Glucose-oxidase, biobleaching, cotton.
Title: Isolation and Characterization of an Inducible Oxidoreductase from Effluent Soil and Its Application for Textile Bio Bleaching for Environment Conservation
Author: Desai U.R, Desai P.V
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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