Isolation and Characterization of Organo-phosphorous Degrading Bactreium Isolated from Agricultural Soil

KARUNAKARAN, S.DHANASEKARAN, V.INDHU, J.JOSEPHINE SAGAYAM, A.STEPHY MARY

Abstract: Organophosphorous pesticides are the world’s most widely used pesticides in agriculture. Exposure of these pesticides and its metabolities have been related to a variety of nerve disorders in humans. Microbial degradation is considered to be a cost effective method for decontamination of toxic organophosphorous pesticides from the environment. In this study we isolated the bacteria from different agricultural field soil to degrade the Organophosphorous pesticides (Monocrotophos, Dichlorovos, chloropyrifos, diozinon) as a soul carbon source. And the results of biochemical tests shows that bacillus sp. Proteus sp. Serratia sp.effectively degrade the organophosphorus pesticides. 16s rRNA Gene sequencing results show that bacillus sp is responsible for degrading MCP Pesticide than others. Ussing thin layer chromatography technique find out the RF values that confirms the genus. It is evident from the results that the isolated bacillus sp could be used for bioremediate the area contaminated with organophosphorous pesticides. Thus we can remediate the environmental pollution.

Keywords: Organophosphorous Degrading Bactreium Isolated From Agricultural Soil, Isolation and Characterization, metabolities.

Title: Isolation and Characterization of Organo-phosphorous Degrading Bactreium Isolated from Agricultural Soil

Author: KARUNAKARAN, S.DHANASEKARAN, V.INDHU, J.JOSEPHINE SAGAYAM, A.STEPHY MARY

International Journal of Interdisciplinary Research and Innovations

ISSN 2348-1218 (print), ISSN 2348-1226 (online)

Research Publish Journals

Vol. 3, Issue 3, July 2015 – September 2015

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Isolation and Characterization of Organo-phosphorous Degrading Bactreium Isolated from Agricultural Soil by KARUNAKARAN, S.DHANASEKARAN, V.INDHU, J.JOSEPHINE SAGAYAM, A.STEPHY MARY