Abstract: Lipases (E.C. 3.1.1.3) are a class of serine hydrolase which belongs to the α/β hydrolases super family. Triacylglycerol acyl esters hydrolase are carboxyl esterase that catalyses both hydrolysis and synthesis of ester formed from glycerol. Lipase enzyme that catalyzes the hydrolysis of stored triacylglycerol releasing di and monoglycerol.
The project deals with the screening of lipase producing organisms. Using Staphylococcus aureus isolated from oil spilled soil, the production of lipase was attempted along with its purification and characterization studies. When different carbon and nitrogen sources are supplemented in the culture medium glycerol, starch, peptone, potassium nitrate found to be the best. When cultivated at optimal temperature and pH of 37°C and 7.5 the lipase produced maximum lipase activity. The organic nitrogen source (peptone) was found to be best lipase enhancing element. The enzyme was partially purified using 60% ammonium sulphate precipitation.
Keywords: Lipase, Staphylococcus aureus, fermentation, optimization.
Title: Isolation and Identification of Lipid Degrading Microorganisms, Optimization of Medium and Partial Purification of the Lipase Enzyme
Author: Anil Khatape, Sambhaji Chavan, Shonak Khade, Dr. Namita Doiphode
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
Research Publish Journals
