Abstract: Oil spill have become a global problem particularly in industrialized and developing countries. Crude oil contamination of the environment is a world-wide threat to environment sustainability and hence a global concern. For this we need to go for the natural solution, i.e. Bioremediation. Organisms were isolated from oil contaminated places i.e. Arabian sea (gateway of India), Gorai creek, Petrol pump, Synthetic soil sample. These isolates were screened using Bushnell Hass agar medium in which diesel oil was used as sole carbon source. The percentage degradation of hydrocarbons estimated using Gravimetric analysis. Time lag and growth pattern of isolates were checked by taking optical density at 530 nm and determining pH at different intervals. 9 different isolates were obtained. The graph of bacterial degradation of oil and the growth curve of isolates was plotted. The bacterial identification was done by performing suitable biochemical test compared with Bergeys Manual. Taxonomic characteristics of these isolates identified them as Bacillus spp., Clostridium spp., Vibrio spp. , Pseudomonas spp. These isolates can be used for degradation of toxic compounds spread during oil spill, land farming, to determine benzene toxicity.
Keywords: Petroleum, Bioremediation, Gravimetric analysis, Bergey’s manual.
Title: Isolation and Identification of Petroleum degrading organisms from oil contaminated water and soil sample
Author: Nikita Nilkanth, Komal Sonne, Ekata Koyande
International Journal of Interdisciplinary Research and Innovations
ISSN 2348-1218 (print), ISSN 2348-1226 (online)
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