Abstract: To explore the effect of different ratio of Phenylalanine and Aspartic acid on longetivity and also quantify its effect at cellular and genetic level of C.elegans. Different ratio of phenylalanine and aspartic acid in ratio of 2:1, 1:2 and 1:1 were assessed. Treated C.elegans were compared with the negative control that was established by culturing normal C.elegans alone. For studying the behavioral assays, different parameters like brooding assay, lifespan assay and lethality assay were evaluated. Cellular toxicities was calculated by MTT assay. Genotoxicity assay indicated the impact on DNA fragmentation. Compared to the normal group, treated group exhibited reduced fecundity rate when treated with 1:2 ratio of Phe:Asp followed by 2:1 and 1:1 ratio. Moreover, these results were in consonance with the results obtained from longetevity assay, whereas the results obtained from lethality assay and MTT assay exhibited that 2:1 ratio of Phe:Asp was more toxic than 1:2 ratio followed 1:1 ratio. DNA fragmentation was maximum in 2:1 ratio of Phe:Asp treated group. Aspartame exhibited apoptotic effect on C.elegans. Aspartic acid component of aspartame reduced the fecundity rate, whereas phenyalalanine component showed enhanced toxicity when compared to aspartic acid in lethality, MTT and DNA fragmentation assay. Results indicated that prolonged use of aspartame as an artificial sweetener may be genotoxic causing deleterious effects on the users.
Keywords: Aspartame, Phenylalanine, Aspartic acid, MTT, DNA fragmentation.
Title: Long-term impact of Artificial Sweetener on Caenorhabditis elegnas
Author: Largee Biswas, Moushumi Das, Anita K. Verma
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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