Abstract: synthetic dyes pollution spreading everywhere, isolation and identification of dye degrading bacterial strains is a difficult process, it need several methods, screening, identification by biochemical and molecular methods, In this direction we isolated three strains of bacteria which were capable of maximum decolorisation and degradation of Reactive red-11 a synthetic dye, derived from the naphthalene as raw material, contains more than one sulphonic acid groups confirms the recalcitrant and xenobiotic character of dye. Work is proceeded with isolating novel dye degrading bacterial strains by various samples, through that efficient bacterial strains were isolated namely- Enterococcus casseliflavus CMGS-1, Lysinibacillus boronitolerans CMGS-2, Klebsiella oxytoca CMGS-3, were selected dye efficient when results were analyzed and through 16srRNA sequencing analysis method and sequence deposited in GenBank with an accession number-, KT602856, KT602857, KT602858 and also organism deposited in the IMTECH (Chandigarh) with an MTCC num-12538, 12531 and 12532 respectively.
Keywords: dye degrading bacterial strains, biochemical, molecular methods.
Title: A methodology - for Isolation of dye degrading bacterial strains
Author: Madhuri R. Basutkar
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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