Abstract: Applying chemical fungicides to control soil-born fungi may lead to environmental pollution. In recent years, microorganisms to control plant pathogens, to improve yield and quality of crop. Fungal species belonging to the genus Trichoderma act as biological agent. Genus Trichoderma effective biocontrol agent against fungal, bacterial pathogen. The fungal isolates Trichoderma harzianum (Tr01) and F. oxysporum (Fu04) was also identified through morphological characterization observed under light microscope (10x×40x). The isolates (Tr01, Fu04) were confirmed through genetic DNA isolation & PCR analysis. After that pot culture study was conducted to test the antagonistic potential of Tr01 against Fu04. Healthy seeds of maize (Zea maize L.) were used in this experiment, They are three different substrates (Rice bran, Black gram bran ,Sugarcane baggase )and soil, water are used in the following ratio of 1:1:2. Nine treatments are T1 Control, T2 Trichoderma harzianum, T3 Fusarium oxysporum , T4Trichoderma harzianum + Ricebran, T5Trichoderma harzianum +Rice bran + Fusaium oxysporum,T6 Trichoderma harzianum +Blackgram bran , T7 Trichoderma harzianum + Blackgram bran +Fusarium oxysporum, T8Trichoderma harzianum+Sugarcane baggase ,T9Trichoderma harzianum + Sugarcane baggase + Fusariumoxysporum.In pot culture after 45 DAI highest plant length for T2 (80cm), highest fresh weight for T6 (0.070), highest dry weight for T2 (0.020) ,highest chlorophyll content T4(9.8695).
Keywords: Biocontrol, Trichoderma harzianum, Fusarium oxysporum, Antagonistic effects, organic substrates.
Title: MASSMULTIPLICATION OF TRICHODERMA HARZIANUM USING AGRICULTURAL WASTE AS A SUBSTRATE FOR AGAINST FUSARIUM OXYSPORUM IN MAIZE PLANTS (ZEA MAYS L.)
Author: Pavithra. R, Lalitha.S
International Journal of Interdisciplinary Research and Innovations
ISSN 2348-1218 (print), ISSN 2348-1226 (online)
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