Abstract: Medium formulation using low cost substrates and suitable culture conditions to optimize protease production were studied by Streptomyces sp. The fermentation medium for the production of alkaline protease by Streptomyces sp. LCJ17A was optimized with glucose as a carbon source, casein as a nitrogen source and red gram husk as an inducer. The optimized values showed that glucose at 3.5 g/L enhances the yield of protease up to 68.3±1.2 U/mL, casein at 0.5 g/L maximizes the protease production up to 71.72±1.2 U/mL and red gram husk at 2.5 g/L yields 73.02±2.1 U/mL. The protease production was 2.5 fold higher compared to that obtained using the unoptimized medium. Through statistical optimization by using Response Surface Methodology, the enzyme yield was further increased to 88.2±0.9 U/mL. This result indicates that the use of glucose, casein and red gram husk as suitable substrates for maximum protease yield and the enzyme activity from the cheaper source enhances the stability of the protease enzyme for industrial applications.
Keywords: Alkaline protease, Starch casein broth, submerged fermentation, Inducer, optimum culture conditions and Streptomyces sp. LCJ17A.
Title: Medium formulation and optimization for improving protease production by Streptomyces sp. LCJ17A
Author: Parthasarathy. M, Joel Gnanadoss. J
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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