Abstract: Asparagus officinalis L. is a herbaceous plant belongs to liliaceae family with semi-erect and branched articulated branches and thick and white root, between 30 and 100 cm in length and 0.7 to 1 cm in thickness and dioecious perennial herb and is native to the Asia, Africa and Europe. This study highlights micropropagation of Asparagus officinalis L. through tissue culture techniques. Asparagus officinalis L. seeds were first washed throughly under running tap water for two hour. And then seed were soaked with distilled water for 24 hours. Seeds were then rinsed using 70%, 50%, 20% and 10% sodium hypochlorite followed by distilled water. This series of seed sterilization was done sequentially. Subsequently seeds were rinsed with 70% ethanol and finally with distilled water prior to culture. Seeds were cultured on Murashige and Skoog, 1962 (MS) basal medium containing 8% agar technical and 30% sucrose. In vitro seed germination was observed within two weeks. Complete plantlets were obtained after four weeks of culture. Explants such as stem and root will later be transferred onto MS medium supplemented with various concentrations of plant growth regulator such as Benzylaminopurine (BAP) and Napthalene Acetic Acid (NAA) to see further organogenesis response. The study showed that in vitro propagation of Asparagus officinalis L. to develop new plantlets was successfully obtained.
Keywords: Asparagus officinalis L., micropropagation, in vitro, plantlets, plant growth regulators.
Title: Micropropagation of Asparagus officinalis L. (Garden Asparagus) In Vitro
Author: Rasad, F. M., Hasbullah, N. A., Azis, N. A., Daud, N. F., Lassim, M.M
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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