Molecular Characterization of Bacteria Isolated From Groundnut (Arachis Hypogaea L.) Root Nodules

Dr. Abdelmalik O. A. Idris, Prof. Awad M. Abdel Rahim, Prof. Abdelmoniem E. Suliman, Prof. Michael Gottfert

Abstract: This study was carried out to characterize three strains of bacteria isolated from groundnut grown in different soils in the Sudan using 16SrRNA gene sequences. The analysis of the sequences revealed that all the three isolates and the reference strains are Klebsiella spp. with similarity ranging from 98% to 99%. However when the sequences were aligned, it was found that there are differences between the different isolated and the reference strains in three positions namely 867, 955 and 964. The differences in the three positions resulted in differences in the translated amino acids of the sequences of the genes also in three positions 289, 319 and 322.

Keywords: Endophytic, Isolates, Klebsiella, Rhizobia, Sequences, Strain.

Title: Molecular Characterization of Bacteria Isolated From Groundnut (Arachis Hypogaea L.) Root Nodules

Author: Dr. Abdelmalik O. A. Idris, Prof. Awad M. Abdel Rahim, Prof. Abdelmoniem E. Suliman, Prof. Michael Gottfert

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

Research Publish Journals

Vol. 3, Issue 1, January 2015 - March 2015

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Molecular Characterization of Bacteria Isolated From Groundnut (Arachis Hypogaea L.) Root Nodules by Dr. Abdelmalik O. A. Idris, Prof. Awad M. Abdel Rahim, Prof. Abdelmoniem E. Suliman, Prof. Michael Gottfert