Abstract: The isolation of stably transfected cell lines suitable for the manufacturing of biotherapeutic protein products such as monoclonal antibodies, is a challenging process and depends on the identification of high expressing clone. This phenomenon involves transgene amplification and maintenance of the expressing clones over numerous generations. Maintenance of cell line and stable protein expression is always depending on the nature of genomic environment at the site of transgene integration. Epigenetic mechanism leads to variable expression and silencing of the transgene. One of the major limitation of introduction of foreign gene into chromosome is, their expression appears to vary with chromosomal site of integration. There are certain elements present in the genomic environment called as chromatin function modifying elements. These elements have ability to negate chromatin insertion site position effect and to express and maintain the stable monoclonal antibody expression in mammalian host. Upon stable cell line generation, chromosomal integration site of the vector DNA has major impact on transgene expression. Here in the current article, different genome integration elements such as Ubiquitous chromatin opening elements (UCOE), stabilizing anti repressor elements (STAR), Chicken hypersensitive site 4 (cHS4), CCCTC binding factor (CCTF), Scaffold \Matrix Attachment Region (S\MAR) are considered.
Keywords: Genome integrating elements, Expression vector, Monoclonal antibody.
Title: Monoclonal Antibody Expression Enhancement by Insertion of Chromatin Modifying Insulator Elements in Mammalian Expression Vector
Author: Mrs. Sharvari Joshi, Dr. Kiran Mangaonkar, Dr. Archana Krishnan
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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