Abstract: The emergence of multiple antibiotic resistances in bacteria and the indiscriminate use of antibiotics contribute to the dissemination of resistant pathogens in the hospital and environment which may cause problems in therapy and is a serious public health issue. This study was conducted to determine the incidence of Pseudomonas aeruginosa isolates in clinical and environmental samples as well as to determine the antimicrobial susceptibility pattern of these isolates to some conventional antibiotics. A total of ninety six (96) samples were collected over a period of one month which includes fifty (50) clinical samples comprising of thirty (30) urine samples collected with sterile urine containers and twenty (20) ear swabs and forty-six (46) environmental samples comprising of twenty three (23) toilet seat swabs and twenty three (23) sink swabs were all collected using sterile cotton swab sticks. A selective medium, Cetrimide agar was used for the isolation of Pseudomonas aeruginosa from the specimens. After 24 hours incubation, there was presence of green, greenish-yellow colonies indicating the probable presence of Pseudomonas aeruginosa. Fifty-one (51) isolates were gotten which include twelve (12) urine isolates (12.50%), sixteen (16) ear swab isolates (16.67%), twelve (12) sink swab isolates (12.50%) and eleven (11) toilet swab isolates (11.45%). The presence of Pseudomonas aeruginosa was confirmed with the following biochemical tests, Gram staining, catalase test and oxidase test. Antibiotic sensitivity testing was carried out as well using Modified Kirby Bauer (Disc diffusion method). The antibiotics used were Amoxicillin clavulanate (30ug), Cefotaxime (25ug), Imipenem (10ug), Ofloxacin (5ug), Gentamycin (10ug), Nalidixic acid (30ug), Nitrofurantoin (300ug), Cefuroxime (30ug), Ceftriaxone sulbactam (45ug), Ampiclox (10ug), Cefexime (5ug) and Levofloxacin (5ug). This study showed the presence of multi-drug resistant (MDR) Pseudomonas aeruginosa from both clinical and environmental samples, with the clinical samples having a higher prevalence of multi-drug resistance compared to environmental samples. The results revealed that the isolates were more resistant to Amoxicillin clavulanate, Ampiclox and Cefexime; however, Imipenem could be the best drug of choice for the treatment of MDR Pseudomonas aeruginosa in South-eastern Nigeria.
Keywords: bacteria, resistance, hospital, environment, urine, Pseudomonas aeruginosa.
Title: MULTI-DRUG RESISTANT PROFILE OF CLINICAL AND ENVIRONMENTAL ISOLATES OF Pseudomonas aeruginosa FROM A TEACHING HOSPITAL AT AMAKU, ANAMBRA STATE, NIGERIA
Author: Afunwa Ruth Asikiya, Odiari Chineze Esther, Nwofia Martin Chukwunonso, Gbadamosi Francis Ayodele
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
Vol. 12, Issue 2, April 2024 - June 2024
Page No: 27-36
Research Publish Journals
Website: www.researchpublish.com
Published Date: 07-June-2024
