Abstract: Escherichia coli (E. coli) exposed to 1-3mM Hydrogen peroxide (H2O2) undergo killing which is induced by DNA damage. Oxidative stress mediated DNA damage can be repaired via DNA repair pathways like base excision repair (BER) and homologous recombination repair. In this study, we have investigated the role of nucleotide excision repair (NER) in survival to oxidative killing. E. coli uvrA mutant showed higher sensitivity to killing by H2O2. Exposure to 3mM H2O2 for 90 minutes led to 900-fold higher killing in uvrA compared to the wild type strain. The sensitivity of uvrA strain was observed across different conditions such as minimal nutrient condition and low aeration. Availability of nutrients and extent of aeration during H2O2 challenge both affected the magnitude of sensitivity but did not alter the sensitivity pattern. This study provides evidence that NER plays an important role in the survival of E. coli under high level of oxidative stress.
Keywords: Escherichia coli, H2O2, Nucleotide Excision Repair, oxidative stress, uvrA.
Title: Nucleotide excision repair is important for survival of hydrogen peroxide mediated killing in Escherichia coli
Author: Amruta Dhawale, Archana Rath
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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