Abstract: Gene silencing by siRNA (short interfering RNA) represents a valuable tool for basic research in biology and proved to have a major impact on medical sciences. Using siRNA is one of the important techniques that can be used to study gene function and its effects on the cells. There are many techniques that can be used to transfect gene specific siRNAs into the cells but some cells are resistant to most of these methods. The present study aimed at optimising the transfection of the mantle cell lymphoma Jeko-1 cell line, which are reported to be difficult to transfect, with siRNA. Two techniques were used: electroporation and nucleofection. The results showed that the use of both electroporation at 220V and nucleofection with solution T result in the highest transfection efficiency and lower cytotoxicity and therefore can be used for the successful transfection of JEKO-1 cells.
Keywords: short interfering RNA (siRNA), transfection efficiency and lower cytotoxicity.
Title: Optimising the Transfection of Mantle Cell Lymphoma Cell Lines with siRNA
Author: Mohammad Al-Jallal, Muhaid Al-Nujidi
International Journal of Healthcare Sciences
ISSN 2348-5728 (Online)
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