Abstract: The production, extraction and optimization of extracellular α-amylase enzyme from some fungal species isolated from five different soil sites in Saudi Arabia. Six fungal species (Aspergillus flavus, A. niger, A. terrus, Cladosporium herbarum, Cunninghamella echinulate and Penicillium italicum) constituting 26 colonies were isolated from the five tested soil samples. The highest significant value of extracellular α-amylase (64.78 unit/ml) was showed in the culture filtrate of the A. niger. Optimization of some nutritional and physical factors in the basal medium in order to intensify the production of A. niger extracellular α-amylase was carried out. The highest productivity of A. niger α-amylase (74.56 and 76.63 unit/ml) occurred on fructose and potassium nitrate as carbon and inorganic nitrogen sources, respectively. The optimum pH and temperature for the maximum productivity of extracellular α-amylase activities were 7.0 and 20 °C, respectively.
Keywords: Fungi, Alpha amylase, Aspergillus, Isolation, Optimization.
Title: Optimization of Alpha Amylase Enzyme Produced by Some Fungal Species Isolated from Saudi Arabian Soil
Author: Khulood S. Alnahdi, Neveen S. Geweely
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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