Abstract: The aim of the study was to extract the Bacteriocin from Staphylococcus aureus and optimize its production using different components and conditions. The research was also designed to know the effect of extracted Bacteriocin on different pathogenic bacteria. The production of Bacteriocin at the commercial level is very cost effective and limited. As many bacteria have the ability to produce Bacteriocin. Staphylococcus aureus was used as a source for the production of Bacteriocin. The study was designed to isolate Staphylococcus aureus from skin followed by the extraction of Bacteriocin. The effect of extracted Bacteriocin was checked on various pathogenic bacteria such as Pseudomonas, E.coli, Listeria, Lactobacillus, Klebsiella and also optimization of the process of Bacteriocin production was performed. The optimization parameters used were temperature, pH, different carbon sources, nitrogen sources and varying salt concentration. The media containing Glucose as a carbon source, Beef extract as a nitrogen source, having 1% salt concentration at pH 6 and at 32 0C temperature yielded maximum Bacteriocin production. The study concluded that Bacteriocin produced from Staphylococcus aureus is effective against pathogenic bacteria and hence can be used as a food preservative.
Keywords: Bacteriocin, BHIB, Optical density, Aureocins.
Title: Optimization of production of bacteriocin from Staphylococcus aureus
Author: Neha, Dr. Bharti Mittu, Dr. Zarina Begum, Dr. Harjot Pal Kaur
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
Research Publish Journals
