Abstract: Brassica oleracea var. italica or commonly known as broccoli is an edible plant grown in Cameron Highland, Malaysia. This research aims to study organogenesis of broccoli through in vitro culture. The seeds were serially sterilized using 70%, 50%, 20% and 10% sodium hypochlorite. Subsequently, the seeds were sterilized using 70% alcohol. Sterilized broccoli seeds were cultured onto Murashige and Skoog, 1962 (MS) basal medium. In vitro response of explant growth was observed. Seed germination was observed within day 12 of seed culture. Elongation of shoots was observed after three to four week of culture. Complete plantlets were observed after 6 weeks of culture. Further organogenesis response was observed when shoot, stem and root explants of eight-week-old aseptic seedlings were then transferred onto Murashige and Skoog medium added with combination of Naphthalene Acetic Acid (NAA) and Benzylaminopurine (BAP). Complete plant regeneration was successfully achieved when root, stem and leaf aseptic explant was cultured on MS medium supplemented with NAA and BAP plant growth regulators. The experimental design used in this study was Completely Randomized Design (CRD). MS medium supplemented with 1.0 mg/L IBA + 0.5 mg/L BAP found to be the optimum medium for shoot regeneration with 5.367±0.768 shoot per explant. Meanwhile 2.0 mg/L NAA shows the optimum medium for leaf and root explant for root induction producing 7.567±0.498 and 8.767±0.707 roots per explant. Micropopagation of Brassica oleracea var. Italica was successfully achieved through plant tissue culture process and proven to be a good alternative to conventional planting.
Keywords: organogenesis, in vitro, Murashige and Skoog medium, seedlings, plantlets.
Title: Organogenesis of Brassica oleracea var. italica Through In Vitro Culture
Author: Azis, N.A., Hasbullah, N. A., Rasad, F.M., Daud, N. F., Lassim, M.M
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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