Abstract: Histopathological diagnosis of benign prostatic hyperplasia (BPH) and prostatic carcinoma (PC) may be problematic. P63, is confined to basal cells/myoepithelial cells in prostate. Cyclin D1 is expressed in the G1 phase of cell cycle and play important role in regulating the cell cycle and cancer progression and Its over-expression is believed to play a role in tumorigenesis including prostatic carcinoma.
Objectives: This study is to assess the expression of P63 and cyclin D1 in BPH and PC, to examine the correlation between results of expression P63 and cyclin D in such lesions, determine the relation between the immunostaining and histologic grade, stage of PC as well as clinical and radiologic findings.
Material and methods: 50 cases of BPH and 50 cases of PC were obtained by TURP (62 cases) and radical prostatectomy (38 cases). For each case, clinical data and radiographic findings were obtained. All immunohistochemical (IHC) analysis was performed on routinely processed, formalin-fixed, paraffin embedded tissue. Tissue sections were cut at 4 µ and mounted on poly-L-lysine-coated slides. Percentage of positive cells was calculated and positive staining scored as: 1+ (weak)= less than 10%, 2+ (moderate) = 11 to 50% and 3+ (strong) = more than 50% tumor cells stained positive.
Results: For P63; 98% of BPH showed positivity and 96% of PC cases showed negativity. For Cyclin D1; 84% of BPH showed negativity while 90% revealed positivity. Degree of reactivity was increased with high Gleason grade but this correlation is not significant.
Conclusion: p63 and Cyclin D1 were highly expressed in BPH and PC respectively, so they may be a valuable tool in differential diagnosis of BPH versus PC lesions.
Keywords: P63, Cyclin D1, Prostate, BPH, PC.
Title: P63 and Cyclin D 1 Expression in Benign Prostatic Hyperplasia versus Prostatic Adenocarcinoma: A Clinicopathologic, Radiologic, And Immunohistochemical Study
Author: Fahd Al Qahtani, Ihab Shafek Atta, E. A. Mady
International Journal of Healthcare Sciences
ISSN 2348-5728 (Online)
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