Abstract: The traditional and alternative systems of medicine depend on large number of medicinal plants, which possess therapeutic properties. The medicinal plants, Nardostachys jatamansi and Adhatoda vasica are two such pharmacologically important plants widely grown in India, Tropical and Sub- Tropical countries. In view of its medicinal values, this study aimed to analyze the phytoconstituents of methanolic extracts of these plants through phytochemical screening and metabolic fingerprinting which was carried out by HPTLC and GC-MS analysis. Total phenol and flavonoid content was evaluated followed by measurement of antioxidant ability by radical scavenging assessment using 1,1,Diphenyl-2-picrylhydrazyl (DPPH) assay. The preliminary data showed the presence of significant amount of flavonoid, phenols and radical scavenging capability comparable to standard antioxidant Ascorbic Acid. The presence of diverse nature of secondary metabolites in methanolic extracts of these plants was confirmed by HPTLC analysis, whereas the GC-MS analysis revealed the presence of at least 23 and 24 metabolites in N. jatamansi and A. vasica respectively. These metabolites consisted of fatty acids, sesquiterpenes, alkane hydrocarbons and esters known to have potent pharmacological effects. Therefore, the present study demonstrates the pharmacological importance of these plants that can be exploited in conventional therapeutic use.
Keywords: Nardostachys jatamansi, Adhatoda vasica, Phytochemical Analysis, HPTLC, GC-MS.
Title: Phytochemical Screening and Metabolic Profiling of Nardostachys jatamansi and Adhatoda vasica
Author: Rasha Irshad, Rayees. A. Padder, Sayeed Ahmed, Mohammad Husain
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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