Abstract: In this study three bacteria were isolated from groundnut root nodules grown in three regions in the Sudan, El-Obied, El-Gezira and El-Gadarif, DNA was extracted, nifH genes were amplified and sequenced and the sequences were compared with reference strain. The analysis of the sequences of nifH genes revealed that all the isolates and the reference strain are Klebsiella with identity of 99% and ensured the presence of nifH gene in all isolates and the reference strain and there is no difference in the sequences except the sequence of El-Gezira strain was found shorter than the others which led to difference of the nifH gene translated amino acids of this strain compared to the others.
Keywords: endophytes, isolates, nitrogenase, nitrogen fixation, sequencing, strains.
Title: Presence of Nifh Gene in Fast Growing Klebsiella Like Bacteria Isolated From Groundnut Root Nodules in the Sudan
Author: Dr. Abdelmalik O. A. Idris, Prof. Awad M. Abdel Rahim, Prof. Abdelmoniem E. Suliman, Prof. Michael Gottfert
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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