Abstract: Siderophores are small protein molecules that can easily bind to ferric iron. As a chelating agent they transport iron molecules inside the bacterial cell for various biochemical reaction. At present studies characterization and Optimization of few siderophores producing bacteria from the soil samples collected from Salem district, Tamil Nadu. The siderophores production was assayed qualitatively and quantitatively through Chrome Azural S and the results showed positive for the strains PSF01 and PSA02 that grown in succinate medium. Further characterization and optimization results revealed that both the bacterium has the ability to yield siderophores in the optimum condition of PH 6 at temperature 37º c with xylose as a carbon source and NH 4SO4 as a Nitrogen Source. Estimation of siderophore production in different media using maximum % siderophore unit (85%) King’s B Medium Fe content in different media using maximum % of siderophore unit (80%) in Nutrient broth medium. The antagonist activity of the siderophore producing Pseudomonas spp against fungal pathogen Fusarium oxysporum. This result showed that Pseudomonas spp is a good producer of siderophore which can be usefully exploited as a biocontrol agent.
Keywords: Biocontrol, Pseudomonas spp, siderophore, Optimization, Fusarium oxysporum.
Title: Production and optimization of microbial iron chelators (Siderophore) byPseudomonas spp
Author: Nithyapriya. S, Lalitha. S
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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