Abstract: Pectin lyase and polygalacturonase production by fungal strain Aspergillus oryzae was carried out in solid-state fermentation. Aspergillus oryzae produced polygalcturonase (PG) and pectin lyase (PL) on mixture of agro-industrial bioproduct in solid state fermentation. To select the optimum growth condition for maximum enzyme production, Mosambi baggage, wheat bran, Mosambi peel and lemon peel were used as substrate for the solid state fermentation process. These substrates were used as sole constituent of media. The Enzyme (wheat bran) was purified by ammonium sulphate precipitation, dialysis and ion exchange chromatography. In the present studies the main objective was to explore new natural source as a substrate for the production of pectinase. Wheat bran act as a best natural source for the production of enzyme from Aspergillus oryzae. The enzyme was purified up to 67.2% fold successfully. It could be employed for extraction of juices as well as clarification with improved quality.
Keywords: Aspergillus oryzae, polygalacturonase, pectin lyase, agro-industrial bioproduct, solid-state fermentation.
Title: Production and purification of Pectinase by fungal strain in solid-state fermentation using agro-industrial bioproduct
Author: Dange V.U., Harke S.
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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