Abstract: About 31 different moist humus rich soil samples were collected from different locations in and around Egmore, Chennai and preliminarily screened by plate assay using Czapex dox medium without carbon source, instead asparagine is used as carbon source and Phenol red is used as indicator which shows pink holo zone from pale yellow colour indicating production of the asparaginase enzyme. From the screening, eight different isolates were showed positive results indicating pink holo zone around the colony. Among the eight isolate N1 showed maximum zone of 5.3 cm whereas enzyme assay by nesselerisation method also revealed isolate N7 showed higher asparaginase activity which was found to be 5.0622 U/ml . From the slide culture the isolate N1 & N7 is identified as Fusarium sp.
Keywords: Asparaginase, Fusarium sp and Phenol red indicator.
Title: PRODUCTION OF ASPARAGINASE ENZYME BY FILAMENTOUS FUNGI
Author: Monisha. D, Anitha. R, Prabhakaran .M
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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