Abstract: The soil acts as a reservoir for millions of microorganisms. Microorganisms play a vital role in maintaining soil fertility and plant health. They can act as biofertilizers. This study aimed at isolating and characterizing plant growth-promoting bacteria associated with the paddy (Oryza sativa, variety - Sugandha), as well as assessing their ability to promote plant growth. Samples were collected from near river Ganga. Sites were dry field, banana field, wetland and stagnant pond areas. Five sampling sites namely S1 (Banana field soil, Hazipur), S2 (Near Gandhi Setu, wetland area), S3 (Sediment pond), S4 (Vaishali) and S5 ( Sonepur, stagnant pond area) were selected for isolation of microbial strains and total of 111 microbial strains (bacteria) were isolated. The strains were selectively isolated from the population of microbes growing on the nutrient agar medium. Strains which showed the best result were tested for its’ biofertilizer potential. These strains were S1 (C), S2 (F) and S3 (R). The isolate S2 (F) was selected on the basis of their luxuriant growth on NA with yellow colour characteristics of colony. This isolate showed a wide range of temperature for growth between 4ْ C to 55ْ C which suggests its potential as biofertilizer. The isolate was Bacillus although molecular characterization (16s r-RNA studies) was done to confirm the genus and species of the selected isolate. On the basis of BLAST alignment of the sequenced nucleotides the isolate S2 (F) was identified as Brevibacillus borstelensis. The reported literatures suggest that the B. borstelensis acts as PGPR.
Keywords: Microbes, Bacteria, Biofertilizer potential, Oryza sativa, Molecular characterization, Brevibacillus borstelensis.
Title: Screening and Assessment of Brevibacillus Borstelensis (Microbial Type Culture Collection No. 10642) as Potential Biofertilizer and Isolated from Selected Gangetic Region of North Bihar
Author: Nimisha Vatsyayan, Ashok K. Ghosh
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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