Abstract: Tumor-associated macrophages constitute the preponderant inflammatory immune infiltrate in solid tumors and the cytokines released from them possess diversified significance in tumor development. Lung cancer cells have been shown to stimulate the macrophages to release various cytokines like TNF-α and IL-6. TNF-α, in particular, has been shown to cause metastasis in mice, however, the process has not been extensively analyzed in human lung cancers. In order to investigate these responses in human lung cancers, we used human adenocarcinoma cell line A549 and human monocytic cell line THP-1 to study the process in detail. Stimulation of THP-1 cells with the conditioned medium of A549 cells causes the release of TNF-α and the co-culture of the two cell types results in the increased migratory abilities of A549 cells in vitro. Stimulation carried out in the presence and absence of serum established that serum could alleviate the stimulatory effects of A549 cells.
Keywords: Lung cancer, TNF-α, Serum, Metastasis.
Title: Serum alleviates the lung cancer induced inflammatory responses from monocytes
Author: Sajad A. Bhat, Basit A. Shah, Asif Amin
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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