Abstract: To govern the sex of given unknown samples by firstly amplifying the SRY gene, which was required for the testis differentiation, using polymerase chain reaction (PCR) technique and then attaching specific primers. Gel electrophoresis was undertaken for separating DNA fragments and visualisation using UV light to get images of bands for comparison of length and results by DNA analysis through spectrophotometry. In this experiment agarose gel was used because of its simple nature, fastness and nontoxicity. Conclusive results were obtained and the analysis was successful.
Keyword: genotyping, sex determining gene, DNA replication, SRY gene, PCR primers, Spectrophotometry.
Title: Sex Determination Using Polymerase Chain Reaction (Pcr): A Research Report
Author: Kaushal Richa
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
Research Publish Journals
