Abstract: The study was conducted for 42days to evaluate the potency of wet testes of bull (Bos. indicus), boar (Sus. domesticus), bull/mud catfish testes (B. indicus / C. gariepinus) and pawpaw seed powder (Carica papaya) on sex reversal of Nile tilapia (O. niloticus) fry in indoor experimental ponds. The estimates of their efficacy in producing phenotypic males were determined after 4months. A total of 825 fry were randomly allocated to 15 experimental ponds. Phyisco-chemical parameters like temperature, dissolve oxygen, PH were checked weekly while ammonia was checked bi- weekly. The hormonal diets were formulated by adding 17α- methyl-testosterone (MT ) T1 , wet testes from bull (T2), boar (T3), bull/catfish testes (T4) and pawpaw seed powder, PSP( T5) at an inclusion of 0.06g, 17.47g, 25.59g, 20.48g (80:20% ratio), and 20g per kg of feed respectively . The fry were fed at 20% body weight during a 42day feeding trial and a gradual reduction of feed from 10% to 5% during the 4months rearing period. . The results of water parameters showed that there were significant differences among all treatments. The results were within the recommended range for fish rearing. Results from the gonad examination revealed that Nile tilapia fry fed with MT-treated (T1) diet gave the highest percent phenotypic males of 100% while T2, T3, T4 and T5 gave male of 80, 86.7, 83.3% and 73% respectively. The use of wet animal testes and pawpaw seed powder can be a natural alternative which is cheap and readily available and also be of great relevance to organic tilapia production. Production of all male will help to reducing prolific breeding in Nile tilapia, consequently leading to an increase in the sizes of the fish which is excellent for market value.
Keywords: Sex reversal, Oreochromis niloticus, wet testes, 17α- methyl-testosterone.
Title: Sex Reversal of Nile Tilapia (Oreochromis Niloticus) Using 17α-Methyl-Testosterone, Wet Testes from Selected Animals and Plant Extract
Author: 0R0SE, EKINADOSE; WOKOMA, ALELEYE; WOKE,GODFREY NGOZI
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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