Abstract: A simple, rapid, specific and precise HPLC method has been developed for the determination of amino acid (Tryptophan) and ketoanalogue (Calcium-3-methyl-2-oxo-butyrate) in solid dosage form. Separation was achieved with Acclaim, OA, C-18 column having 250 × 4.6 mm i.d, 5 µM particle size analytical column using mixture of acetonitrile, and Methane sulfonic acid solution with isocratic mode. The instrumental settings are at a flow rate of 1.0mL/min, column temperature at 40°C and UV detection at 235 nm. The retention time was found to be about 4 and 9 min respectively. RSD and tailing factor were found to be well within the limit for both the compounds. The percentage contents found by the proposed method were more than 98 % for both the compound. Precision were found 0.10% and 0.30 % respectively. This method was validated and meets the regulatory requirements for precision, specificity and linearity. The proposed method can be used for the quantification of Tryptophan and Ca-3-methyl -2-oxo-butyrate in solid dosage forms.
Keywords: Tryptophan, Valine, Calcium salts, Ketoanalogue, RP-HPLC Method.
Title: Simultaneous Estimation of Tryptophan and Valine by RP- HPLC Method
Author: S.K.Badole, Dr. K.B. L. Shrivastava
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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