Abstract: Present study was focused on to appraise the peroxidase activity and phenolic content in two mosses Atrichum pallidum and Campylopus subfragilis under the different concentrations of two heavy metals Copper and Cadmium for 10, 20 and 30 days. The preferred concentrations for the experiment were 100ppm to 400ppm of Copper and 5ppm to 20ppm of Cadmium. Both the mosses showed significant increase in peroxidase activity and phenolic content when treated with different concentrations of Cadmium and Copper. C. subfragilis showed better survival (increased peroxidase activity) with Cu and the maximum increase was 0.61±0.01Kat/sec/mg/protein in 300ppm till 30th day as compared to A. pallidum in which maximum increase was 0.31±0.05Kat/sec/mg/protein in 300ppm till 20th day. During the analysis of phenols Campylopus subfragilis under Cu stress the maximum increase in content was 28±0.07mg/g/fw in 400ppm on 20th day whereas A. pallidum showed maximum increase up to 9.23±0.005 mg/g/fw in 400ppm on 10th day.
Under Cd stress, C. subfragilis showed maximum increase in peroxidase activity in 10ppm as 0.48±0.004 Kat/sec/mg/protein on 10th day and in A. pallidum as 0.215±0.03 Kat/sec/mg/protein in 5ppm on 10th day, whereas the maximum increase in phenolic content in C. subfragilis in 10ppm was 34±0.03 mg/g/fw on 30th day and in A. pallidum was7.8±0.004 mg/g/fw in 5ppm on 20th day. After the maximum increase in activity as well as content both the taxa showed gradual decline. During AAS analysis the net content of absorbed concentration of Cu in C. subfragilis was 142.1ppm and in A. pallidum was 219.6ppm, and of Cd was 0.80ppm in C. subfragilis and 0.87ppm in A. pallidum. The regeneration was much better in C. subfragilis as compared to A. pallidum. All these observations led to conclusion that C. subfragilis survived better in both the metals (Cu or Cd) as it has absorbed less content of Cu or Cd as compared to A. pallidum. Hence A. pallidum can be used as a good bio-monitor (due to more content absorbed of Cu or Cd) as well as bio-indicator ( due to its sensitive response to metal stress) of heavy metal pollution.
Keywords: Mosses Campylopus subfragilis, Atrichum pallidum, Phytotoxic metals, Copper, Cadium, enzyme peroxidase, secondary metabolite phenols, Impact, AAS.
Title: Studies in Atrichum pallidum and Campylopus subfragilis subjected to Cu and Cd toxicity for evaluation of their role in pollution monitoring
Author: Vibha, Sunita Kapila
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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