Abstract: The aim of this work is to study occurrence of genus Spirulina and biochemical composition of Spirulina subsalsa isolated from various localities of Satara district, Maharashtra. Four species of Spirulina were recorded from the study area viz., Spirulina subsalsa Oersted ex Gomont, Spirulina meneghiniana Zanardini ex Gomont, Spirulina major Kutzing ex Gomont and Spirulina gigantea Schmidle. Out of these Spirulina subsalsa was cultured in conical flasks under lab conditions. Algal biomass was harvested at its exponential phase. Fresh as well as dried biomass was subjected to biochemical analysis. The metabolites considered for biochemical analysis were pigments viz., chlorophyll a, carotenoids and Phycobiliproteins; carbohydrate, protein, lipids, Nitrate reductase (NR) activity, Total flavonoid content (TFC),Total Phenolic content (TPC),Total antioxidant capacity (TAC) and Vitamin C. It showed significant amount of chlorophyll- a i.e. 6.510(mg/ml) with 3.800 (mg/ml) carotenoid content. Total phycobiliproteins recorded was 5.60 (mg/ml) with 0.800 (mg/ml) Phycoerythin, 1.800 (mg/ml) Phycocyanin and 3.00(mg/ml) allophycocyanin respectively. It showed 8.00 % (dry wt.) Carbohydrates, 49.00 % (dry wt.) proteins and 25.00% (dry wt.) lipids which makes it superfood. In addition Total flavonoid content (TFC) recorded was 3.970 (mg./g equivalent of Rutin);Total Phenolic content (TPC) 0.305(mg./g GAE),Total antioxidant capacity (TAC) 0.525(mg./g AAE), and Vitamin C 0.452(mg./g). Spirulina subsalsa have been found with promising biochemical characterization which may be exploited further in future.
Keywords: Biochemical analysis, Cyanobacteria, Satara, Spirulina subsalsa.
Title: Studies on cyanobacteria: Spirulina isolated from Satara district, Maharashtra
Author: Priyadarshani Kamble, Vanita Karande
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
Research Publish Journals
