Abstract: In this work, a general profile of circulating cells (or hemocytes) in the hemolymph of the sand lobster, Thenus orientalis has been studied, which includes total hemocyte count (THC), identification of various hemocyte morphotypes and their relative proportion in circulation (differential hemocyte count or DHC). Mean THC was found to be 3 × 106 cells/ml. Three distinct hemocyte morphotypes were identified in the systemic circulation of the lobster namely, hyaline, semigranular and granular cell types with a relative proportion of 43 %, 30 % and 27 % respectively In addition, this paper also highlight an attempt to isolate different hemocyte morphotypes using percoll by discontinuous density gradient centrifugation. Separation of lobster hemocyte morphotypes through this method yielded partially successful result, in which two bands were obtained. The first band was found in between 20 and 40 % percoll gradient and was populated with large number of hyaline hemocytes and semigranular type 1 cells. The second band appeared in between 40 and 60 % percoll gradient and majorly consisted of granular and semigranular type 3 cells. A minor population of semigranular type 2 cells were seen in both the bands.
Keywords: Hemocytes, THC, DHC, Morphotype separation, Percoll density gradient centrifugation.
Title: STUDIES ON THE PROFILE OF HEMOCYTES WITH AN ATTEMPT TO SEPARATE DIFFERENT HEMOCYTE SUB – POPULATIONS FROM THE HEMOLYMPH OF THE SAND LOBSTER, THENUS ORIENTALIS (LUND, 1793)
Author: Vasudevan Vaishnavi, Periasamy Mullainadhan, Mohammed Farzana Yasmeen
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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