Abstract: In order to determine the percentage of Klebsiella pneumoniae which isolated from the upper and lower bovine respiratory tract, to determine a biofilm formation ability of these isolates, in addition to detection the presence of ecpA gene in these strains using conventional PCR assay. One hundred nasal swabs and same number of lung tissue samples were taken randomly from apparently healthy and diseased cattle that expressed respiratory clinical signs of different ages and gender and they were processed in laboratory of Veterinary Medicine College. The K. pneumoniae biofilm formation ability was detected by spectrophotometric method. Results of bacterial isolation showed that unhealthy animals exposed 7 (3.18%) of K. pneumoniae in lung samples and 4(1.8%) from nasal passages while apparently healthy animals revealed 1 (0.45%) and 3 (1.4%) K. pneumoniae isolates from lung and nasal passages respectively. The biofilm formation result showed that 10 (66.7%) were positive formers while negative formers composed 5(33.3%). Results of PCR assay revealed that 14(93.3%) of K. pneumoniae isolates had ecpA gene while 1(6.7%) of these isolates were lacked this gene.
Keywords: Respiratory tract, Klebsiella pneumoniae, biofilm formation, PCR, ecpA gene.
Title: Study of ecpA prevalence with biofilm formation ability in Klebsiella pneumoniae isolated from Respiratory Tract of Cattle in Certain area of Iraq
Author: Rand T. Yaseen, Mohammed J. Alwan
International Journal of Life Sciences Research
ISSN 2348-3148 (online), ISSN 2348-313X (Print)
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