Abstract: The objective of this study was to isolate and identify the oral microorganisms implicated in oral infections in the population and also to evaluate and classify the effectiveness of common dental medicaments. The study tested the potency of the medicaments against oral microorganisms.
Methods: Bacteriological investigation was done on (140) oral samples. The samples were cultured in aerobic and anaerobic conditions on non-selective and selective media. Standard procedures of bacterial culture and identification were applied. Nine medicaments were utilized to test for sensitivity levels against isolates from the oral cavity.
Results: 12 microbes were identified. Some were pathogenic while others were opportunistic organisms. The highest sensitivity was demonstrated by Acinetobacter calcoaceticus and Proteus mirabilis. The lowest sensitivity was seen in Pseudomonas aeruginosa and Hafnia alvei. The most effective medicament against the isolates was Formocresol and the least effective medicament was Calcium hydroxide. Bacterial Survival Index test was performed to show the sum of colony formation units. The lowest survival rate was found in Formocresol dilutions while the highest survival rate was found in Calcium Hydroxide dilutions indicative of sensitivity and resistance respectively. Restriction enzyme analysis showed varying banding patterns among the DNA of the organisms. Unique bands were seen with varying sizes of 8,000 base pairs to 23,000 base pairs.
Conclusion: The oral microorganisms may have implications on the health of individuals harboring them. Therefore, the knowledge of medicaments and microorganism that inhabit the oral cavity is important in predicting and preventing not only dental diseases but also associated systemic complications.
Keywords: Oral infections, microbes, medicaments, biofilm.
Title: The Biochemical Response of Oral Microorganisms to Common Dental Medicaments in Jamaica
Author: Paul Gyles, Ph.D., Gad Onywere, M.Sc., Phillip Mighty Dental Surgeon (DDS), Patience Bazuaye-Alonge, Ph.D.
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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