Abstract: The continuous activities of companies that deal with substances such as hydrocarbons and their derivatives in the environment are the primary source of pollution in our ecosystem, and they must be eliminated and managed. Biocatalysts secreted by Saccharomyces cerevisiae, Bacillus cereus, and Pseudomonas aeruginosa were identified and characterized in this study. The oil-polluted environment is an ideal habitat for isolating lipase-producing microorganisms. At the University of Port Harcourt, Aluu, and Rupkokwu were chosen as case studies because of their distinctive oily environments for isolating lipase-producing microorganisms. The goal of this investigation was to isolate, screen, identify, and characterize lipase-producing fungi and bacteria from oil-contaminated soil. While screening, inoculation on the spot for primary screening, and Lipase synthesis in submerged fermentation were used. To test the effect of temperature on lipase activity, the reaction was carried out at temperatures ranging from 10 to 90 °C. Pseudomonas aeruginosa produced the most lipase, followed by Bacillus cereus, with a minor amount of lipase in the shape of a zone around the Saccharomyces cerevisiae colony. Pseudomonas aeruginosa exhibited the greatest enzyme activity of 17.19 U/L after 72 hours of inoculation into the culture medium, and the biocatalyst producing temperature conditions were maximized at 37oC. The enzyme activities of the three isolates were 0.5 U/L after 96 hours of inoculation. The lipase was extremely stable in terms of temperature and organic solvent. This one-of-a-kind feature makes it appealing and practical for industrial applications.
Keywords: Bacillus cereus, Biocatalyst, Biomaterial, Environment, Enzymes, Pseudomonas aeruginosa, Saccharomyces cerevisiae.
Title: ISOLATION, SCREENING, AND IDENTIFICATION OF BIOCATALYST FROM BACTERIA AND FUNGI IN OIL POLLUTED SOIL
Author: Ubong U.Isang, Gideon C. Okpokwasili, Francis Ire
International Journal of Engineering Research and Reviews
ISSN 2348-697X (Online)
Research Publish Journals
