Abstract: The present investigation has been done to validate the mutagenic and cytotoxic effect of a neonicotinoid insecticide, Acetamiprid through ames test and root meristem study of Trigonella foenum-graecum. Presoaked seeds of Trigonella foenum-graecum were treated with various concentrations of Acetamiprid viz 15, 30, 45 and 60ppm for 6, 12 and 24 hrs. The treated seeds were washed thoroughly in running tap water and allowed to germinate on moist filter paper in petriplate at 25 ± 1ºc. Cytogenetic damages were analyzed after 120 hrs. The result showed significant decrease (p ˂0.05; p˂0.01) in mitotic index and increased chromosomal aberration and micronuclei. The ames test done at the same concentration to validate the mutagenicity also reflected similar results showing increased number of revertant colonies in TA100, TA 102 , TA 1535. The results were observed to be dose dependent for the selected tester strains. These findings help to validate that the insecticide Acetamiprid is a potential mutagen and also have cytotoxic effect on Trigonella foenum-graecum.
Keywords: Acetamiprid, Ames test, Chromosomal aberration, Trigonella foenum-graecum.
Title: Validation of cytotoxicity and mutagenicity of Acetamiprid through Ames and root meristem study of Trigonella foenum-graecum. L
Author: D. S. Yasodha, Dr. K.M. Umarajan, Dr. S.Malathy
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
Research Publish Journals
